Epidemiological characteristics and risk factors of COPD in people over 60 years old in Chaohu City, Anhui Province / 公共卫生与预防医学

Objective To investigate the epidemiological characteristics of COPD in people over 60 years old in Chaohu City, Anhui Province, and explore the risk factors affecting the occurrence and development of COPD, and to provide a data basis for formulating prevention and control measures of COPD. Methods Permanent residents over 60 years old in Chaohu City were selected as the research subjects. The subjects were selected by multi-stage stratified cluster random sampling method. All research subjects were divided into two different groups according to the diagnostic criteria of COPD and received questionnaire survey and pulmonary function tests. The differences in epidemiological distribution of the two groups were compared by χ2 test. Logistics regression was used to analyze the risk factors affecting COPD. Results A total of 1722 cases were investigated in this study. The prevalence of COPD in people over 60 years old was 13.47%. The average age and body mass index of all research subjects were 70.4±7.5 years old and 20.4±3.5kg/m2, respectively. Among them, 49.25% were males, 20.96% had college degree or above, and 63.88% lived in cities and towns. 23.11% of the subjects still smoked, 32.58% had quit smoking, and 28.16% had a history of passive smoking or were still receiving passive smoking. 16.20% of the subjects had an occupational exposure to dust and particulate matter as coal miners and painters. 51.34% of the subjects had a family history of respiratory diseases. 83.57% of the subjects had other chronic diseases, including coronary heart disease, hypertension, and diabetes. There were 232 cases diagnosed as COPD in this study. All subjects were divided into the COPD group and control group according to the diagnostic criteria of COPD. There were no significant differences between the two groups in body mass index, marital status, education, residence, type of fuel used and family history of respiratory diseases. However, there were significant differences in age, gender, smoking status, occupational dust exposure and chronic disease complications between the two groups. Multivariate logistic regression analysis showed that age (OR70~79 vs. 60~69=2.578, P=0.024; OR≥80 vs. 60~69=4.258, P=0.018), smoking status (ORpassive smoking vs. Active smoking=0.683, P=0.073; ORquit smoking vs. active smoking=0.365, P=0.020; ORNon-smoking vs. active smoking=0.252, P=0.008) and occupational dust exposure (OR=0.158, P=0.010) were the major risk factors for COPD. Conclusion Advanced age, active or passive smoking and occupational dust exposure are important risk factors for COPD.

Norlichexanthone purified from plant endophyte prevents postmenopausal osteoporosis by targeting ER

Although different types of drugs are available for postmenopausal osteoporosis, the limitations of the current therapies including drug resistances and adverse effects require identification of novel anti-osteoporosis agents. Here, we defined that norlichexanthone (NOR), a natural product, is a ligand of estrogen receptor-alpha (ER

Clinical value of 18F-FDG PET/CT imaging in evaluation of antineutrophil cytoplasmic antibody-associated vasculitis / 中华核医学与分子影像杂志

Objective To explore the clinical value of 18F-fluorodeoxyglucose (FDG) PET/CT in assessing antineutrophil cytoplasmic antibody-associated vasculitis (AAV).Methods Fifteen patients (7 males,8 females,age (66±11) years) with AAV between January 2015 and June 2017 were retrospectively analyzed.There were 6 patients diagnosed as granulomatosis with polyangiitis (GPA),7 diagnosed as microscopic polyangiitis (MPA) and 2 diagnosed as eosinophilic granulomatosis with polyangiitis (EGPA).All patients underwent 18 F-FDG PET/CT and the image features were observed and analyzed.The maximum standardized uptake value (SUVmax) of the positive lesion was measured.The relationship between the SUVmax and C reactive protein (CRP) was analyzed with Pearson correlation.The SUVmax and the number of lesion sites were compared by two-sample t test between the CRP-elevated and CRP-normal patients.Results A total of 56 lesions in the 14 of 15 AAV patients were detected by PET/CT.The positive findings distributed in 15 tissues and organs,including the nasopharynxes (n =9),lungs (n =9),kidneys (n =8),spleen (n =6),lymph nodes (n =6),bone marrow (n =4),skin (n =3),prostate (n =2),aortas (n =2),vertebral soft tissues (n =2),orbita (n =1),parotid gland (n =1),thyroid gland (n =1),liver (n =1) and pancreas (n=1).The 60.7％ (34/56) of lesions were clinically unsuspected occult lesions.GPA lesions mainly invaded the nasopharynxes,lungs and kidneys;MPA lesions mainly invaded the kidneys and spleen;EGPA lesions mainly invaded the nasopharynxes,lymph nodes and bone marrow.There was no significant correlation between the level of CRP and the SUVmax of AAV lesions (r=0.462,P＞0.05).No differences in the SUVmax were observed between patients with elevated CRP levels and those with normal CRP levels (t=1.451,P＞0.05).But more lesion sites were observed in patients with elevated CRP (t=3.456,P＜0.05).Conclusions 18F-FDG PET/CT shows positive findings in multiple sites in AAV patients,including clinically unsuspected sites.This imaging technique may be a useful tool for diagnosis and evaluation of AAV.

Secondary metabolites of petri-dish cultured Antrodia camphorata and their hepatoprotective activities against alcohol-induced liver injury in mice / 中国天然药物

Antrodia camphorata, a well-known and highly valued edible medicinal mushroom with intriguing activities like liver protection, has been traditionally used for the treatment of alcoholic liver disease. A. camphorata shows highly medicinal and commercial values with the demand far exceeds the available supply. Thus, the petri-dish cultured A. camphorata (PDCA) is expected to develope as a substitute. In this paper, nineteen triterpenes were isolated from PDCA, and thirteen of them were the unique anthroic acids in A. camphorata, including the main content antcin K, which suggested that PDCA produced a large array of the same anthroic acids as the wild one. Furthermore, no obvious acute toxicity was found suggesting the edible safety of PDCA. In mice alcohol-induced liver injury model, triglyceride (TG), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and malondialdehyde (MDA) had been reduced by the PDCA powder as well as the main content antcin K, which indicated that the PDCA could protect alcoholic liver injury in mice model and antcin K could be the effective component responsible for the hepatoprotective activities of PDCA against alcoholic liver diseases.

Molecular epidemiology and mechanisms of fosfomycin-resistant Escherichia coli producing extended-spectrum β-lactamases isolated from urine / 临床检验杂志

Objective@#To investigate the molecular epidemiology and mechanisms of fosfomycin-resistant Escherichia coli isolates producing extended-spectrum β-lactamases (ESBLs) isolated from urine. @*Methods@#Fosfomycin-resistant phenotypes were screened by drug susceptibility test in ESBLs-producing E.coli stains. The ESBLs gene and fosfomycin resistant gene were amplified by PCR. The molecular typing was analyzed by multiple sequence type (MLST). The transferability of the drug resistant gene was verified by conjugation assays. @*Results@#Among the 308 strains of E.coli isolated from urine, there were 168 (54.54%) ESBLs producing strains and 18 (10.71%, 18/168) fosfomycin resistant strains. In the drug resistant genes, the incidence of bla SHV was about 88.9% (16/18), bla CTX-M was 77.8% (14/18), bla TEM-208 was 5.6% (1/18), bla TEM-1b was 61.1% (11/18) and fosA3 was 83.3% (15/18). The main MLST genotype was ST131. The conjugation test confirmed the transferability of resistance in fosA3 positive strain. @*Conclusion@#The fosfomycin resistant rate of ESBLs producing E.coli in urethral infection was in a low level. The fosA3 gene was the main mechanism of fosomycin resistance and located on the conjugation plasmids, which should be the main node of horizontal gene transmission. We need to pay great attention to this resistant gene.

Research on displacement model of urine flow rate meter calibration device based on regression analysis / 医疗卫生装备

Objective To determine the relationship between the theory and actual displacement of the urine flow rate meter calibration device gear pump, and to construct a model between actual and theory displacement, in order to obtain actual displacement via theory displacement. Methods The weight of the standard water flow in each flow rate was measured by B2000S digital scales,and the scatter plot between the theory displacement and actual displacement was drawn to find the best fitted curve;then the MATLAB Curve Fitting Tool was used to obtain the best quasi relational equation. Results The error between the fitted displacement and the actual displacement of the gear bump was 1.494% in maximum and-0.010% in minimum, far less than 5% of the requirements for urinary flow meter measurement. Conclusion Based on regression analysis, the relationship between the theoretical displacement and actual displacement of the flow rate meter calibration device is established,the fitting error is small,and the method is simple and easy to achieve.

Detection and Clinical Significance of Treg and Th1,Th2 Cells in Peripheral Blood of Patients with Immune Thrombocytopenia / 现代检验医学杂志

Objective To detect the level of CD4+CD25+FoxP3+ (Treg) and Th1,Th2 cells in peripheral blood of patients with immune thrombocytopenia and discuss its clinical significance.Methods Flow cytometry was used to detect the percentage of Treg cells and Th1,Th2 cells in peripheral blood of 27 patients with ITP.25 healthy volunteers were detected as control group.Results The percentage of Treg cells in the peripheral blood of patients with ITP was (2.20 ± 0.93)％,which significantly decreased compared with the healthy control group (2.99 ± 0.45) ％ (t =3.820,P＜0.01) respectively,and the levels of Th1,Th2 cells were not statistically different between the two groups (tTh1 =0.655,tTh2 =0.467,all P＞ 0.05).Conclusion Treg cells in patients with ITP were decreased,and its important role in the development of ITP deserves further study.

Clinical cognition of anterolateral femoral skin flap with high cutantous artery branches / 中华显微外科杂志

Objective To recognize the relationship between high cutantous artery branches and descending branch of lateral femoral circumflex artery artery,and to investigate the blood supply of anterolateral thigh flap in clinical.Methods Retrospective analysis 152 cases of anterolateral thigh flap from November,2003 to December,2016.It contains cutting with descending branchs in 99 cases,cutting with lateral branchs in 43 cases,the union of them in 8 cases and high cutantous artery branches in 2 cases.Results The flaps survival in 147 cases,cutting necrosis in 3 cases and partly necrosis in 2 cases.Descending and lateral branchs were both dominance neurovascular bundle of vastus lateralis muscle,independently or commonly dominate the skin of anterolateral thigh,occurrence rate was 33.5％.Conclusion The descending branch of lateral femoral circumflex artery include lateral branch,all of which are the neurovascular bundles,supply the anterolateral thigh muscle,and divid into some perforator branches crossing the musle to the anterolateral thigh skin subsequently,which constitute "the anatomical functional unit".High cutantous artery branche is the one of all of the branches,its origin lies hight and arise from lateral branch.

Value of 18F-FDG PET/CT in differentiating tuberculous and cancerous diffuse peritoneal lesions / 天津医药

Objective To study the 18F-FDG PET/CT features of tuberculous and malignant diffuse peritoneal lesions and to discuss the diagnostic value of 18F-FDG PET/CT in diagnosing and differentiating the lesions. Methods The 18F-FDG PET/CT features of 72 patients with tuberculous peritonitis,28 primary serous papillary carcinoma of the peritoneum and 135 peritoneal metastases confirmed by clinic and/or histopathology, were retrospectively reviewed. The peritoneal thickening features of tuberculous and malignant peritoneal lesions were observed. The maximal standardized uptake value (SUVmax) of peritoneal lesions and ascites, ascites SUVmax/liver SUVmax (T/NT) were compared between tuberculous peritonitis and cancerous peritonitis. The ROC curve was used to analyze the diagnostic efficiency of T/NT, SUVmax of peritoneal lesions and ascites. Results The typical 18F-FDG PET/CT features of tuberculous peritonitis were uniformity thickening of parietal peritoneum, mesenteric and omental stain like changes, widely and even distribution of the peritoneal 18F-FDG, while the cancerous peritonitis was obvious uneven thickening of parietal peritoneum, mesenteric and omental nodules and pie-shape changes, uneven distribution of the peritoneal 18F-FDG. The degree of 18F-FDG uptake was increased in all peritoneal lesions, and there was no significant difference between the tuberculous group (SUVmax=10.53±5.44) and the cancerous group (SUVmax=11.45±6.78, t=1.017, P>0.05). The 18F-FDG concentration in malignant ascites (SUVmax=1.88±0.65,T/NT=0.73± 0.18) was obvious higher than that of tuberculous ascites (SUVmax=1.67±0.69,T/NT=0.57±0.27, t=2.243 and 5.045,both P<0.05). The area under the ROC curve of T/NT, SUVmax of ascites, SUVmax of peritoneal lesions were 0.707, 0.593, 0.536, respectively. Conclusion The 18F-FDG PET/CT imaging can reflect the morphology and metabolic changes of peritoneal lesions. It is important to combine the SUVmax of ascites in order to improve the efficiency of diagnosing the diffuse peritoneal diseases.

Detection and the Significance of SOCS-1 Gene Methylation Status in Peripheral Blood of Systemic Lupus Erythematosus Patients / 现代检验医学杂志

Objective To investigate the relationship between the systemic lupus erythematosus (SLE)and the SOCS-1 gene methylation status of the peripheral blood DNA,to provide the basis for diagnosis and treatment of systemic lupus erythema-tosus.Methods Blood samples of SLE patients (27 cases)and healthy group (19 cases)in January 2015 to April were col-lected and the DNA were extracted.Using polymerase chain reaction combining DNA agarose gel electrophoresis to detect the SOCS-1 gene methylation status.Results In patients with systemic lupus erythematosus SOCS-1 gene complete methyl-ation accounted for 44% (12/27),incomplete methylation accounted for 56% (15/27).In healthy group SOCS-1 gene com-plete methylation accounted for 74% (14/19)and incomplete methylation accounted for 26% (5/19).The rate of complete methylation of SOCS-1 gene of SLE patients was lower than that of healthy group (χ2=3.88,P=0.049).Conclusion SLE patients may have lower SOCS-1 gene methylation status in the peripheral blood DNA,which is worth for further study.

Research Progress on the Role of Chromatin Remodeling Factor BRG1 in Acute Myeloid Leukemia / 中国实验血液学杂志

BRG1 (Brahma-related gene 1, BRG1) is the ATPase subunit of SWI/SNF chromatin remodeling complexes, which plays an important role in cell cycle regulation, DNA repair and tumor development. Unlike the evidence as tumor suppressor genes in the past reports, latest researches show that BRG1 plays an important role in sustaining the growth of leukemia cells in acute myeloid leukemia, and these effects on normal hematopoietic stem cells are dispensable. Further studies of the role and mechanism of BRG1 in acute myeloid leukemia will contribute to the development of a new and promising targeted therapy strategy. This article reviews the role of BRG1 on leukemia cells and leukemia stem cells in AML and discusses the related mechanism, which providing some reference for the targeted treatment strategy of AML.

The role of low dose CT as part of the same 18F-NaF PET/CT in detecting bone metastases in lung cancer / 实用医学杂志

Objective Retrospectively evaluate low dose computed tomography (CT) as part of a combined 18F-NaF positron emission tomography (18F-NaFPET/CT) examination in lung cancer patients suspected of bone metastases. Methods 118 of 122 lesions with increased uptake of 18F-NaF were assessed. Characteristics of bone metastases on CT images were reviewed by radiologists. Results 27 of 47 metastases presented as sites of increased uptake with corresponding lytic or sclerotic changes on low dose CT. Other 20 show normal or non-specific appearing bone on CT. Most benign lesions (67 of 71,94%)have a benign appearance on low-dose CT. Conclusions Low dose CT images were useful in precisely diagnosing bone metastasis. Negative low dose CT appearance to the abnormal foci on PET may be a reliable sign of metastases.

Prevalence of Hepatitis B Virus Infection in Systemic Lupus Erythematosus Patients / 现代检验医学杂志

Objective To investigate the prevalence of Hepatitis B virus(HBV)infection in systemic lupus erythematosus (SLE)patients.Methods HBV and HCV serological tests performed in the Gulou Hospital Affiliated to Medical College of Nanjing University from January 2010 to March 2015 were retrospectively investigated for analysis HBV and HCV infection rate.The clinical testing data of 866 SLE inpatients (SLE group)from January 2010 to March 2015 were retrospectively in-vestigated for analysis HBV and HCV infection rate.The serological tests performed in 1 795 health examination people (Control group)to estimate the HBV and HCV infection ratein general population using ELISA.Compare the difference of HBV/HCV infection rate between SLE group and Control group.Results In the SLE group,17 patients were HBsAg posi-tive,the positive rate was 1.96%.In the control group HBsAg postive 204 patients,the positive rate was 11.4%,there were significant differences between these two groups (χ2 =67.81,P <0.0001).The HBsAg positive rate was lower in male SLE patients compared with controls (5.26% VS 13.9%,χ2 =4.58,P <0.05).For the female SLE patients,the HBsAg positive rate was significantly lower than the control (1.64% VS 8.12%,χ2 = 35.65,P <0.0001).The HBsAg positive rate was lower in SLE group compared with control group among different age groups,and the difference was significant in 21~30, 31~40 and 41~50 age group (χ2 =21.86,22.78,20.36;all P <0.001).There had no statistical difference between SLE and control group for the HBsAb positive rate(Total,χ2 =0.50,P =0.48;Male,χ2 =0.12,P =0.73;Female,χ2 =2.00,P =0.16).Conclusion The prevalence of HBV infection in SLE patients was lower than general population.

Experimental study on multiple tracers PET/CT in the differentiation of C6 glioma from different inflammation / 中华核医学与分子影像杂志

Objective To investigate the value of 18F-FDG,11C-MET and 11C-CHO PET/CT in the differentiation of C6 glioma from different kinds of inflammation in experimental rat models.Methods (1) A total of 48 male SD rats were randomly divided into 6 groups by the random number table:group 1 and 2 consisted of 8 rats bearing both C6 glioma and turpentine oil-induced acute inflammation;group 3 and 4 consisted of 8 rats bearing both C6 glioma and turpentine oil-induced chronic inflammation;group 5 and 6 consisted of 8 rats bearing both C6 glioma and BCG-induced granuloma.(2) 18F-FDG and 11C-MET PET/CT were performed on rats of group 1,3 and 5;18F-FDG and 11C-CHO PET/CT were performed on rats of group 2,4 and 6.The lesion-to-muscle ratios and tumor selectivity index (SI) were calculated.(3)After the PET/CT imaging,the lesions were excised.Immunohistochemical staining was used to demonstrate the situation of Glut-1,HIF-1α and CD98.(4)Two-sample t test,Nemenyi test and nonparametric Kruskal-Wallis H test were used for statistical analyses.Results (1) 18 F-FDG and 11 C-MET uptake in C6 glioma were higher than those in different inflammatory tissues(t--1.425-3.901,all P＜0.05).The 11 C-CHO uptake among different lesions were not significant (t =0.031-3.901,all P＞0.05).In group 1 and 5 models,SIMET(4.22±2.96 and 4.89±2.08) was significantly higher than SIFDG(1.77±0.86 and 1.72±0.77;t =2.717and 2.490,both P＜0.05);but iu group 3 models,SIMET(3.84±2.71) was not significantly higher than SIFDG(2.28± 1.14;t =2.082,P＞0.05).(2) Immunohistochemical study showed that there were significant differences in the expression of HIF-1 α,CD98 among different lesions (H =17.810,26.540,both P ＜ 0.05),and no significances of expression of Glut-1 among different lesions (H=5.940,P＞0.05).Nemenyi test showed that there was significant difference for CD98 expression between C6 glioma and acute inflammation,C6 glioma and granuloma (x2=5.504,9.345,both P＜O.05),and for HIF-1α and CD98 expression between C6 glioma and chronic inflammation (x2 =-5.938,2.128,both P＜0.05).Conclusions Compared with 18F-FDG and 11 C-CHO,11 C-MET has better tumor specificity.11 C-CHO PET/CT is not suitable for the differentiation of tumor and inflammation because of its lowest specificity.

Study of the brain glucose metabolism in different stage of mixed-type multiple system atrophy / 中华核医学与分子影像杂志

Objective To investigate the brain glucose metabolism in different stage of mixed-type multiple system atrophy (MSA).Methods Forty-six MSA patients with cerebellar or Parkinsonian symptoms and 18 healthy controls with similar age as patients were included.According to the disease duration,the patients were divided into three groups: group 1 (≤ 12 months,n=14),group 2 (13-24 months,n=13),group 3 (≥ 25 months,n =19).All patients and controls underwent 18F-FDG PET/CT brain imaging.To compare metabolic distributions between different groups,SPM 8 software and two-sample t test were used for image data analysis.When P＜0.005,the result was considered statistically significant.Results At the level of P＜0.005,the hypometabolism in group 1 (all t＞3.49) was identified in the frontal lobe,lateral temporal lobe,insula lobe,anterior cingulate cortex,caudate nucleus and anterior cerebellar hemisphere.The regions of hypometabolism extended to posterolateral putamen and part of posterior cerebellar hemisphere in group 2 (all t＞3.21).In group 3,the whole parts of putamen and cerebellar hemisphere were involved as hypometabolism (all t＞4.08).In addition to the hypometabolism regions,there were also stabled hypermetabolism regions mainly in the parietal lobe,medial temporal lobe and the thalamus in all patient groups (all t＞3.27 in group 1,all t＞3.02 in group 2,all t＞3.30 in group 3).Conclusions Disease duration is closely related to the FDG metabolism in the MSA patients.Frontal lobe,lateral temporal lobe,anterior cingulate cortex and caudate nucleus can be involved at early stage of the disease.Putaminal hypometabolism begins in its posterolateral part.Cerebellar hypometabolism occurs early at its anterior part.Besides,thalamus shows hypermetabolism in the whole duration.18F-FDG metabolic changes of brain can reflect the development of mixed-type MSA.

Clinical and neuroimaging analysis of 4 patients with corticobasal degeneration syndrome / 中华神经科杂志

Objective To investigate clinical and imaging features of corticobasal degeneration syndrome (CBDS). MethodsA clinical, imaging and therapeutic analysis of 4 cases of clinically diagnosed with corticobasal degeneration was conducted. Results Asymmetric parkinsonism was the first symptom in all 4 cases who lacked of response to levodopa. Other symptoms including limb apraxia and anarthria occurred in all 4 cases,myoclonus occurred in three,dementia occurred in two,and involuntary movement occurred in one. All the patients had asymmetric frontoparietal cortical atrophy in the contralateral to the dominantly affected limbs on MRI.Asymmetric hypometabolism of the frontoparietal cortex and basal ganglia was observed on 18 F-FDG PET in all 4 cases.ConclusionsThe clinical features of CBDS are asymmetric parkinsonism,dementia,apraxia,anarthria,myoclonus,and involuntary movement.Brain MRI and 18 F-FDG PET are helpful to diagnosis of CBDS.There is no effective treatment for CBDS at this time.

The correlations between 18F-FDG uptake and a variety of tumor biological indicators in C6 glioma rats with radiotherapy / 中华核医学杂志

ObjectiveTo study the relationship between 18F-FDG uptake and tumor cell density,glucose transporter expression,cellular proliferation and angiogenesis before and after radiotherapy in C6 glioma rats.MethodsThirty C6 glioma-bearing male SD rats were randomly divided into three groups:A,B and C ( 10 rats in each group).Two weeks later,18F-FDG PET/CT was performed in group A.In groups B and C,18 F-FDG PET/CT was performed at 48 h and 1 week after radiotherapy,respectively.The ratio of SUVmax of tumor to muscle (T/M) was calculated.HE staining,immunohistochemical staining and Western blot were used to measure tumor cell density,Ki67 labeling index ( LI),microvessel density ( MVD),Glut-1 and VEGF expression quantitatively.The one-way analysis of variance and bivariate correlation analysis were used to compare the changes of each indicator and evaluate the correlation between T/M and biological indicators,respectively.Results Significant differences of T/M,tumor cell density,Ki67 LI,MVD,Glut-1 and VEGF among groups A,B and C were observed ( F =6.77,60.66,104.56,95.49,9.13,24.48,respectively,all P ＜0.05).Least significant difference (LSD) test showed that there was no significant difference between group A and B in T/M,tumor cell density and Ki67 LI ( 10.86 ± 3.31,730.50 ± 78.93,20.02 ± 2.14 vs 9.23 ± 4.56,672.70 ± 92.98,18.56 ± 2.26).However,the indicators of group C (5.16 ± 2.52,355.60 ± 72.62,7.81 ± 1.76 ) were significantly decreased compared with those of groups A and B (all P ＜0.05 ).MVD and Glut-1 expression of group B increased slightly compared with those of group A ( 19.50 ± 1.96,0.20 ± 0.09 vs 17.90 ± 2.02,0.15 ± 0.04),but the difference was not statistically significant.Nevertheless,the two indicators were significantly decreased in group C ( 8.40 +1.84 and 0.07 ±0.06,P ＜0.05).VEGF expression in group B (0.42 ±0.13) was significantly higher than that in groups A and C (0.17 ±0.04 and 0.16 ± 0.09) ( both P ＜ 0.05 ).The changes of T/M were positively correlated with the changes of tumor cell density between groups A and B ( r =O.81,P ＜ 0.05 ).Changes of T/M were positively correlated with the changes of tumor cell density,Ki67 LI,MVD and Glut-1 between groups A and C (r =O.83,0.71,0.68,0.62,all P ＜ 0.05 ).ConclusionsThe changes of 18 F-FDG uptake in C6 glioma rats were only correlated to the changes of tumor cell density at 48 h after radiotherapy.However,the changes of 18F-FDG uptake closely correlate to the changes of a variety of biological indicators at 1 week post radiotherapy.

Fluorodeoxyglucose positron emission tomography/computed tomography and magnetic resonance imaging of uterine leiomyosarcomas: 2 cases report / 中华医学杂志(英文版)

Uterine leiomyosarcoma is an uncommon malignant neoplasm of smooth muscle origination and is associated with a poor prognosis. We report two cases of uterine leiomyosarcoma that presented with pulmonary metastases. 2-deoxy-2-(¹⁸F)fluorodeoxyglucose (FDG) positron emission tomography (PET)/computed tomography (CT) was performed to identify the primary carcinoma and found the focus located in the uterus. The follow-up magnetic resonance imaging (MRI) confirmed the diagnosis was uterine leiomyosarcoma.

Whole Body ~(18)F-FDG PET/CT Imaging for Detecting Primary Tumors of Cervical Metastatic Adenocarcinoma from Unknown Primary Site / 天津医药

Objective:To retrospectively evaluate whole body ~(18)F-FDG PET/CT in depicting the primary tomors in cervical metastatic adenocarcinoma of unknown primary site.Methods:Seventeen patients were included and underwent whole body PET/CT imaging.The primary tumors and metastases were detected by visual evaluation and measurements of standardized uptake values.The PET/CT impression was verified by histopathology and(or)follow-up.Results:In 17 patienis,primary tumors of 13 patients were confirmed by pathological or clinical follow-up.Among them,8 were in the lung,1 in breast,1 in renal,2 in colon and 1 in cervix.Among the other 4 cases,3 cases were correctly diagnosed as malignant lymphoma finally by PET/CT instead of metastatic adenoearcinoma previously.The sensitivity,specificity and accuracy of PET/CT in were 92.3%,75.0% and 88.2%,identifying primary tumors respectively.After PET/CT examination,10 patients(58.8%)were detected additional metastatic lesions which were missed previously.Conclusion:Whole body ~(18)F-FDG PET/CT may be of relatively higher value in identifying the primary tumor of cervical metastatic adenocarcinoma from unknown primary site.It also may display tumor involvement extent.

Correlation study between ~(18)F-FDG uptake and hypoxia inducible factor-1α level,microvessel density in human gliomas / 中华核医学杂志

Objective To investigate the correlation between ~(18)F-fluorodeoxyglucose (FDG) uptake and hypoxia inducible factor1α (HIF-1α) level,microvessel density (MVD) in human gliomas.Methods ~(18)F-FDG PET scan was performed preoperatively in 41 patients with gliomas (including 23 highgrade and 18 low-grade tumors).The ratios of maximum standardized uptake value(SUV_(max))between tumor (T)and contralateral white matter (WM) were calculated (T/WM).Immunohistochemical stain methods were used to evaluate the level of HIF-1α and measure the MVD in tumors.Correlation analysis between SUV_(max) of T/WM and HIF-1α level,MVD wag performed.The t-test,one-way ANOVA test,Spearman rank correlation and Wilcoxon signed-rank test were calculated using SPSS 11.5 software.Results (1)The SUV_(max) of T/WM,HIF-1α level and MVD in high-grade and low-grade tumors groups were 3.39±1.43,95.7% and 44.13±16.1 vs 1.46±0.55.55.6% and 18.83±7.07,respectively.The difierences of SUV_(max) of T/WM,HIF-1α level and MVD between two groups were statistically significant (t=-5.921,z=-3.938,t=-6.745,all P＜0.05).(2)Among 41 gliomas,the strong positive expression of HIF-1α was observed in 8,mederate in 9,weak in 15 and negative expression was found in 9,SUV_(max) of T/WM and MVD increased with increasing HIF-1α level.The differences of SUV_(max) of T/WM and MVD among 4 different groups were statistically significant (F=7.41,P＜0.05).(3) The MVD of all gliomas was ranged from 9.76 to 94.52,which correlated with SUV_(max) of T/WM(r=0.759,P＜0.05).Conclusions The SUV_(max) of T/WM correlates with HIF-1α level and MVD in gliomas.Therefore,~(18)F-FDG PET provides preoperatively a noninvasive assessment of hypoxia or angiogenesis in human glionma.